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              肝竇內(nèi)皮細(xì)胞,HHSEC細(xì)胞

              • 產(chǎn)品型號(hào):
              • 產(chǎn)品時(shí)間:2024-08-13
              • 簡(jiǎn)要描述:肝竇內(nèi)皮細(xì)胞,HHSEC細(xì)胞 ,慧穎細(xì)胞庫(kù)提供HHSEC的價(jià)格、來(lái)源、 培養(yǎng)條件以及注意事項(xiàng)等?;鄯f細(xì)胞庫(kù),擁有完善的細(xì)胞庫(kù)管理體系,供應(yīng)400多種類細(xì)胞株細(xì)胞系:國(guó)內(nèi)/外優(yōu)質(zhì)細(xì)胞供應(yīng),種類齊全,質(zhì)量保證,,100%放心免費(fèi)售后!自細(xì)胞庫(kù)成立至今,供應(yīng)于全國(guó)各省市地區(qū),擁有北京上海各地中科院研究所,復(fù)旦,瑞金,上藥所等各大小型院校及企業(yè)。
              • 產(chǎn)品簡(jiǎn)介

              肝竇內(nèi)皮細(xì)胞,HHSEC細(xì)胞 全國(guó)統(tǒng)一價(jià)格,全國(guó)各地均可發(fā)貨,遠(yuǎn)到香港、澳門、福建、新疆、內(nèi)蒙古等地。

              產(chǎn)品名稱:HHSEC細(xì)胞

              中文名稱:肝竇內(nèi)皮細(xì)胞

              種屬:人

              來(lái)源:美國(guó) Sciencell

              生長(zhǎng)狀態(tài):貼壁生長(zhǎng)|懸浮生長(zhǎng)|半貼壁半懸浮生長(zhǎng)

              細(xì)胞形態(tài):上皮樣|成纖維樣|圓形|不規(guī)則

              培養(yǎng)條件:DMEM|1640|MEM|IMDM+10% FBS

              細(xì)胞規(guī)格:5-10×105細(xì)胞量

              特征特性:  肝竇內(nèi)皮細(xì)胞是肝臟非實(shí)質(zhì)細(xì)胞的主要細(xì)胞群,由肝竇內(nèi)皮細(xì)胞構(gòu)成的肝竇壁是全身毛細(xì)血管壁中*缺乏基膜的毛細(xì)血管窗孔,是肝竇內(nèi)皮細(xì)胞特征性的結(jié)構(gòu)。肝竇內(nèi)皮細(xì)胞在調(diào)節(jié)肝竇血流與周圍組織的物質(zhì)交換中起有效的中樞性的作用,因此肝竇內(nèi)皮細(xì)胞對(duì)于維持正常的肝功能起十分重要的作用。

              庫(kù)存狀態(tài):現(xiàn)貨

              運(yùn)輸方式:運(yùn)輸方式分為新鮮運(yùn)輸和凍存管運(yùn)輸兩種運(yùn)輸方式

              貨期:新鮮運(yùn)輸需要1-2周,凍存管運(yùn)輸?shù)男枰?-3天

              慧穎細(xì)胞庫(kù)提供的所有細(xì)胞僅供科研使用

              原代細(xì)胞的培養(yǎng)是指直接從機(jī)體取下細(xì)胞、組織和器官后立即進(jìn)行培 養(yǎng);因此,較為嚴(yán)格地說(shuō)是指成功傳代之前的培養(yǎng),此時(shí)的細(xì)胞保持原有細(xì)胞的基本性質(zhì),如果是正常細(xì)胞,仍然保留二倍體數(shù)。不同的原代細(xì)胞傳代次數(shù)不同,具體需咨詢客服人員或者自行查閱 相關(guān)資料。

              細(xì)胞株是通過(guò)選擇法或克隆形成法從原代培養(yǎng)物或細(xì)胞系中獲得的具有特殊性質(zhì)或標(biāo)志的培 養(yǎng)細(xì)胞。從培養(yǎng)代數(shù)來(lái)講,理論上可培養(yǎng)到40-50代。

              客戶應(yīng)具有一定細(xì)胞培養(yǎng)知識(shí)與經(jīng)驗(yàn),并具備基本培養(yǎng)條件(細(xì)胞培養(yǎng)實(shí)驗(yàn)室,無(wú)菌操作臺(tái)、CO2培養(yǎng)箱,可拍照倒置顯微鏡等),并按說(shuō)明書(shū)要求準(zhǔn)備好相應(yīng)的無(wú)菌的培養(yǎng)基、血清、雙抗、細(xì)胞培養(yǎng)瓶、培養(yǎng)板等。如果因客戶缺少基本培養(yǎng)經(jīng)驗(yàn)和培養(yǎng)條件而導(dǎo)致細(xì)胞死亡或污染,不在我司售后責(zé)任之內(nèi)。

              相關(guān)肝竇內(nèi)皮細(xì)胞,HHSEC細(xì)胞熱賣產(chǎn)品:

              骨、關(guān)節(jié)、軟組織和皮膚系統(tǒng)Bones, joints,soft tissue and skin system

              BJ細(xì)胞,人原代成纖維細(xì)胞         5 × 105         1-2weeks

              HSF細(xì)胞, 人皮膚成纖維細(xì)胞         5 × 105         1-2weeks

              FLS細(xì)胞, 成纖維樣滑膜細(xì)胞         5 × 105         1-2weeks

              A-431細(xì)胞,人皮膚鱗癌細(xì)胞     5 × 105         1-2weeks

              SK-MEL-1 細(xì)胞,人皮膚黑色素瘤細(xì)胞         5 × 105         1-2weeks

              A875細(xì)胞,人黑色素瘤細(xì)胞     5 × 105         1-2weeks

              A-375細(xì)胞, 人惡性黑色素瘤細(xì)胞     5 × 105         1-2weeks

              HaCaT細(xì)胞, 人永生化表皮細(xì)胞         5 × 105         1-2weeks

              hFOB1.1細(xì)胞,人永生化成骨細(xì)胞     5 × 105         1-2weeks

              hFOB 1.19細(xì)胞,SV40轉(zhuǎn)染人成骨細(xì)胞    5 × 105         1-2weeks

              HT-1080細(xì)胞,人纖維肉瘤細(xì)胞  5 × 105         1-2weeks

              MG-63細(xì)胞,人成骨肉瘤細(xì)胞     5 × 105         1-2weeks

              Saos-2細(xì)胞, 人成骨肉瘤細(xì)胞     5 × 105         1-2weeks

              TE671 subline No.2 人橫紋肌肉瘤細(xì)胞         5 × 105         1-2weeks

              U266細(xì)胞,人骨髓瘤細(xì)胞         5 × 105         1-2weeks

              HOS細(xì)胞,人骨肉瘤細(xì)胞          5 × 105         1-2weeks

              U-2 OS細(xì)胞,人骨肉瘤細(xì)胞          5 × 105         1-2weeks

              h-BMSC細(xì)胞,人骨髓間質(zhì)細(xì)胞   5 × 105         1-2weeks

              呼吸系統(tǒng)respiratory system

              16HBE細(xì)胞,人支氣管上皮樣細(xì)胞     5 × 105         1-2weeks

              95-D細(xì)胞,人高轉(zhuǎn)移肺癌細(xì)胞         5 × 105         1-2weeks

              H322細(xì)胞,肺癌細(xì)胞         5 × 105         1-2weeks

              A549細(xì)胞,人肺癌細(xì)胞     5 × 105         1-2weeks

              H1299細(xì)胞,人肺癌細(xì)胞     5 × 105         1-2weeks

              PC9細(xì)胞,  人肺癌細(xì)胞     5 × 105         1-2weeks

              PC14細(xì)胞, 人肺癌細(xì)胞     5 × 105         1-2weeks

              SPCA-1細(xì)胞,人肺腺癌細(xì)胞         5 × 105         1-2weeks

              Calu-3細(xì)胞,人肺腺癌細(xì)胞         5 × 105         1-2weeks

              LTEP-a-2細(xì)胞,人肺腺癌細(xì)胞         5 × 105         1-2weeks

              MRC-5細(xì)胞,人胚肺成纖維細(xì)胞         5 × 105         1-2weeks

              WI-38細(xì)胞, 人胚胎肺成纖維細(xì)胞     5 × 105         1-2weeks

              A549(成瘤)細(xì)胞,         人非小細(xì)胞肺癌細(xì)胞     5 × 105         1-2weeks

              NCI-H358細(xì)胞,人非小細(xì)胞肺癌細(xì)胞     5 × 105         1-2weeks

              NCI-H1650 細(xì)胞, 人非小細(xì)胞肺癌細(xì)胞     5 × 105         1-2weeks

              NCI-H1975細(xì)胞,人非小細(xì)胞肺腺癌細(xì)胞         5 × 105         1-2weeks

              NCI-N838細(xì)胞, 人非小細(xì)胞肺癌細(xì)胞     5 × 105         1-2weeks

              SK-MES-1細(xì)胞, 人肺鱗癌細(xì)胞         5 × 105         1-2weeks

              NCI-H226 細(xì)胞,人肺鱗癌細(xì)胞         5 × 105         1-2weeks

              NCI-H520細(xì)胞,人肺鱗癌細(xì)胞         5 × 105         1-2weeks

              NCI-H460細(xì)胞, 人大細(xì)胞肺癌細(xì)胞         5 × 105         1-2weeks

              NCI-H661細(xì)胞, 人大細(xì)胞肺癌細(xì)胞         5 × 105         1-2weeks

              泌尿系統(tǒng)urinary system

              293  人胚腎細(xì)胞     5 × 105         1-2weeks

              293A  人胚腎細(xì)胞     5 × 105         1-2weeks

              293F人胚腎細(xì)胞     5 × 105         1-2weeks

              HEK293  人胚腎細(xì)胞     5 × 105         1-2weeks

              293XL-hTLR7 人胚腎細(xì)胞     5 × 105         1-2weeks

              293XL-hTLR9 人胚腎細(xì)胞     5 × 105         1-2weeks

              293-mTLR5     人胚腎細(xì)胞     5 × 105         1-2weeks

              293 xhTLR7      人胚腎細(xì)胞     5 × 105         1-2weeks

              293 xhTLR9      人胚腎細(xì)胞     5 × 105         1-2weeks

              293H         人胚腎細(xì)胞     5 × 105         1-2weeks

              293FT       人胚腎細(xì)胞-用于慢病毒包裝        5 × 105         1-2weeks

              293TN      人胚腎細(xì)胞--用于慢病毒包裝       5 × 105         1-2weeks

              293T/17   人胚腎細(xì)胞-用于慢病毒包裝        5 × 105         1-2weeks

              293LVT     人胚腎細(xì)胞--用于慢病毒包裝       5 × 105         1-2weeks

              293T         人胚腎細(xì)胞(SV40T基因修飾) 5 × 105         1-2weeks

              LNCaP clone FGC     人前列腺癌細(xì)胞     5 × 105         1-2weeks

              DU 145     人前列腺癌細(xì)胞     5 × 105         1-2weeks

              786-O       人腎透明細(xì)胞癌細(xì)胞     5 × 105         1-2weeks

              Caki-1       人腎透明細(xì)胞癌皮膚轉(zhuǎn)移細(xì)胞     5 × 105         1-2weeks

              G401         人腎癌Wilms細(xì)胞          5 × 105         1-2weeks

              HK-2          人腎皮質(zhì)近曲小管上皮細(xì)胞         5 × 105         1-2weeks

              ADPC        人雄激素依靠性前列腺癌細(xì)胞     5 × 105         1-2weeks

              RWPE-1    人正常前列腺上皮細(xì)胞         5 × 105         1-2weeks

              T24  人膀胱癌細(xì)胞         5 × 105         1-2weeks

              EJ     人膀胱癌細(xì)胞         5 × 105         1-2weeks

              5637         人膀胱癌細(xì)胞         5 × 105         1-2weeks

              ScaBER     人膀胱鱗癌細(xì)胞     5 × 105         1-2weeks

              內(nèi)分泌系統(tǒng)endocrine system

              BT-474     人乳腺導(dǎo)管癌細(xì)胞         5 × 105         1-2weeks

              435(中科:MDA-MB-435S )     人乳腺導(dǎo)管癌         5 × 105         1-2weeks

              HS-578T   人乳腺癌細(xì)胞         5 × 105         1-2weeks

              MCF-7      人乳腺癌細(xì)胞         5 × 105         1-2weeks

              MDA-MB-231  人乳腺癌細(xì)胞         5 × 105         1-2weeks

              T47D         人乳腺癌細(xì)胞         5 × 105         1-2weeks

              LLC   人乳腺癌細(xì)胞         5 × 105         1-2weeks

              MCF 7       人乳腺癌細(xì)胞         5 × 105         1-2weeks

              MDA-MB-231  人乳腺癌細(xì)胞         5 × 105         1-2weeks

              SKBR3       人乳腺腺癌細(xì)胞     5 × 105         1-2weeks

              MCF 10A  人正常乳腺上皮細(xì)胞     5 × 105         1-2weeks

              SW579      人甲狀腺鱗癌細(xì)胞         5 × 105         1-2weeks

              TT     人髓狀甲狀腺腫瘤細(xì)胞         5 × 105         1-2weeks

              腦和神經(jīng)系統(tǒng)The brain and nervous system

              A172         人膠質(zhì)母細(xì)胞瘤細(xì)胞     5 × 105         1-2weeks

              BE(2)-M17        人神經(jīng)母細(xì)胞瘤細(xì)胞     5 × 105         1-2weeks

              IMR-32     人神經(jīng)母細(xì)胞瘤細(xì)胞     5 × 105         1-2weeks

              SH-SY5Y   人神經(jīng)母細(xì)胞瘤細(xì)胞     5 × 105         1-2weeks

              SK-N-SH    人神經(jīng)母細(xì)胞瘤細(xì)胞     5 × 105         1-2weeks

              SHG-44     人膠質(zhì)瘤細(xì)胞         5 × 105         1-2weeks

              HS 683      人腦膠質(zhì)瘤細(xì)胞     5 × 105         1-2weeks

              U-373MG          人腦神經(jīng)膠質(zhì)瘤細(xì)胞     5 × 105         1-2weeks

              U251         人神經(jīng)膠質(zhì)細(xì)胞瘤細(xì)胞         5 × 105         1-2weeks

              U-87 MG  人腦星形膠質(zhì)母細(xì)胞瘤細(xì)胞         5 × 105         1-2weeks

              生殖系統(tǒng)reproductive system

              C-33A       人宮頸癌細(xì)胞         5 × 105         1-2weeks

              Ca Ski      人宮頸癌細(xì)胞         5 × 105         1-2weeks

              Hela229   人宮頸癌細(xì)胞         5 × 105         1-2weeks

              HeLa         人宮頸癌細(xì)胞         5 × 105         1-2weeks

                       人胚胎成纖維細(xì)胞         5 × 105         1-2weeks

              HEC-1-B   人子宮內(nèi)膜腺癌細(xì)胞     5 × 105         1-2weeks

              Ishikawa  人子宮內(nèi)膜癌細(xì)胞         5 × 105         1-2weeks

              SKOV3       人卵巢癌細(xì)胞         5 × 105         1-2weeks

              HO-8910  人卵巢癌細(xì)胞         5 × 105         1-2weeks

              SK-OV-3    人卵巢腺癌細(xì)胞     5 × 105         1-2weeks

              OVCAR-3 人卵巢腺癌細(xì)胞     5 × 105         1-2weeks

              JEG3         人絨毛膜癌細(xì)胞     5 × 105         1-2weeks

              Ms751      人子宮頸表皮癌細(xì)胞     5 × 105         1-2weeks

              Siha  人子宮頸鱗癌細(xì)胞         5 × 105         1-2weeks

              KLE   人子宮內(nèi)膜腺癌細(xì)胞     5 × 105         1-2weeks

              消化系統(tǒng)digestive system

              PANC-1細(xì)胞,人胰腺癌細(xì)胞        5 × 105         1-2weeks

              SW1990細(xì)胞,       人胰腺癌細(xì)胞         5 × 105         1-2weeks

              BxPC-3細(xì)胞,人胰腺腺癌細(xì)胞    5 × 105         1-2weeks

              chang liver細(xì)胞,  人張氏肝細(xì)胞         5 × 105         1-2weeks

              HIC-2細(xì)胞,  人小腸癌細(xì)胞         5 × 105         1-2weeks

              HCT-8細(xì)胞,人回盲腸癌細(xì)胞      5 × 105         1-2weeks

              SW620[SW-620] 細(xì)胞,人結(jié)腸癌細(xì)胞          5 × 105         1-2weeks

              HCT-116細(xì)胞,人結(jié)腸癌細(xì)胞      5 × 105         1-2weeks

              COLO 205 細(xì)胞,人結(jié)腸癌細(xì)胞     5 × 105         1-2weeks

              RKO細(xì)胞,人結(jié)腸腺癌細(xì)胞 5 × 105         1-2weeks

              LoVo細(xì)胞,人結(jié)直腸腺癌細(xì)胞    5 × 105         1-2weeks

              HT-29細(xì)胞,  人結(jié)直腸腺癌細(xì)胞         5 × 105         1-2weeks

              Caco-2細(xì)胞,人結(jié)直腸腺癌細(xì)胞         5 × 105         1-2weeks

              SW480細(xì)胞,人結(jié)直腸腺癌細(xì)胞         5 × 105         1-2weeks

              SW620細(xì)胞,人結(jié)直腸腺癌細(xì)胞         5 × 105         1-2weeks

              SW837細(xì)胞,人結(jié)直腸腺癌上皮細(xì)胞         5 × 105         1-2weeks

              DLD-1細(xì)胞, 人結(jié)直腸腺癌上皮細(xì)胞         5 × 105         1-2weeks

              KYSE30細(xì)胞,人食管鱗癌細(xì)胞    5 × 105         1-2weeks

              KYSE-150 細(xì)胞,人食管鱗癌細(xì)胞         5 × 105         1-2weeks

              KYSE-450細(xì)胞,人食管鱗癌細(xì)胞          5 × 105         1-2weeks

              TE-1 細(xì)胞,人食管癌細(xì)胞     5 × 105         1-2weeks

              ECA109細(xì)胞,人食管癌細(xì)胞        5 × 105         1-2weeks

              EC9706細(xì)胞,人食管癌細(xì)胞        5 × 105         1-2weeks

              MH97H細(xì)胞,人高轉(zhuǎn)移潛能肝癌細(xì)胞株   5 × 105         1-2weeks

              HCCLM3細(xì)胞, 人高轉(zhuǎn)移肝癌細(xì)胞         5 × 105         1-2weeks

              SMMC-7721細(xì)胞,人肝癌細(xì)胞    5 × 105         1-2weeks

              BEL-7404 細(xì)胞,人肝癌細(xì)胞         5 × 105         1-2weeks

              Hep G2細(xì)胞,人肝癌細(xì)胞    5 × 105         1-2weeks

              Hep-3B細(xì)胞,人肝癌細(xì)胞    5 × 105         1-2weeks

              BEL-7402 細(xì)胞,人肝癌細(xì)胞         5 × 105         1-2weeks

              BEL-7404 細(xì)胞,人肝癌細(xì)胞         5 × 105         1-2weeks

              SNU-739  細(xì)胞,人肝癌細(xì)胞         5 × 105         1-2weeks

              HepG2細(xì)胞,人肝癌細(xì)胞     5 × 105         1-2weeks

              97L細(xì)胞,人肝癌細(xì)胞  5 × 105         1-2weeks

              HuH-7細(xì)胞, 人肝癌細(xì)胞     5 × 105         1-2weeks

              QGY-7703細(xì)胞,人肝癌細(xì)胞        5 × 105         1-2weeks

              HOC細(xì)胞,   肝卵圓細(xì)胞     5 × 105         1-2weeks

              L-02(HL-7702) 細(xì)胞, 人正常肝細(xì)胞         5 × 105         1-2weeks

              QSG-7701細(xì)胞,人正常肝細(xì)胞         5 × 105         1-2weeks

              GES-1細(xì)胞,  人胃上皮細(xì)胞         5 × 105         1-2weeks

              MGC-803細(xì)胞,人胃癌細(xì)胞         5 × 105         1-2weeks

              MKN-45細(xì)胞,人胃癌細(xì)胞  5 × 105         1-2weeks

              AGS細(xì)胞, 人胃癌細(xì)胞     5 × 105         1-2weeks

              AZ-521細(xì)胞,人胃癌細(xì)胞    5 × 105         1-2weeks

              Hs 746T細(xì)胞,人胃癌細(xì)胞  5 × 105         1-2weeks

              NUGC-3細(xì)胞,人胃癌細(xì)胞  5 × 105         1-2weeks

              SNU-638細(xì)胞,人胃癌細(xì)胞 5 × 105         1-2weeks

              NCI-N87細(xì)胞,人胃癌細(xì)胞  5 × 105         1-2weeks

              HGC-27細(xì)胞,人胃癌細(xì)胞(未分化)        5 × 105         1-2weeks

              BGC-823  細(xì)胞,人胃腺癌細(xì)胞     5 × 105         1-2weeks

              SGC-7901  細(xì)胞,人胃腺癌細(xì)胞     5 × 105         1-2weeks

              GBC-SD細(xì)胞,人膽囊癌細(xì)胞        5 × 105         1-2weeks

              QBC939細(xì)胞,人膽管癌細(xì)胞         5 × 105         1-2weeks

              血液和淋巴系統(tǒng)Blood and lymphatic system

              6TCEM細(xì)胞,人T細(xì)胞白血病細(xì)胞    5 × 105         1-2weeks

              JURKAT細(xì)胞,人T淋巴細(xì)胞白血病細(xì)胞株        5 × 105         1-2weeks

              CCRF-CEM細(xì)胞,  人急性淋巴細(xì)胞白血病T淋巴細(xì)胞     5 × 105         1-2weeks

              MOLT-4細(xì)胞,人急性淋巴母細(xì)胞白血病細(xì)胞   5 × 105         1-2weeks

              REH 細(xì)胞,急性淋巴細(xì)胞白血病         5 × 105         1-2weeks

              supb15細(xì)胞,急淋白血病細(xì)胞系         5 × 105         1-2weeks

              K-562細(xì)胞,  人慢性髓原白血病細(xì)胞         5 × 105         1-2weeks

              K562細(xì)胞,人慢性髓性白血病細(xì)胞    5 × 105         1-2weeks

              HL-60細(xì)胞,  白血病細(xì)胞     5 × 105         1-2weeks

              TF-1細(xì)胞,人血液白血病細(xì)胞     5 × 105         1-2weeks

              HSPC-1細(xì)胞,人造血干細(xì)胞         5 × 105         1-2weeks

              Daudi細(xì)胞,  人Burkitt淋巴瘤細(xì)胞    5 × 105         1-2weeks

              NAMALWA細(xì)胞,  人Burkitt淋巴瘤細(xì)胞    5 × 105         1-2weeks

              Raji細(xì)胞 人Burkitt淋巴瘤細(xì)胞    5 × 105         1-2weeks

              RAMOS細(xì)胞,人B淋巴細(xì)胞瘤細(xì)胞   5 × 105         1-2weeks

              HUVEC[HUV-EC-C] 細(xì)胞,    人臍靜脈內(nèi)皮細(xì)胞         5 × 105         1-2weeks

              MPCs細(xì)胞,  單核巨噬細(xì)胞         5 × 105         1-2weeks

              THP-1細(xì)胞,  急性單核白血病細(xì)胞     5 × 105         1-2weeks

              U937細(xì)胞,   人組織細(xì)胞淋巴瘤細(xì)胞         5 × 105         1-2weeks

              HCAEC細(xì)胞,人冠狀動(dòng)脈內(nèi)皮細(xì)胞    5 × 105         1-2weeks

              HemSc細(xì)胞,血管瘤干細(xì)胞         5 × 105         1-2weeks

                           人淋巴細(xì)胞     5 × 105         1-2weeks

              循環(huán)系統(tǒng)circulatory system

              HUV-EC細(xì)胞,人臍靜脈內(nèi)皮細(xì)胞       5 × 105         1-2weeks

              眼、耳、鼻、喉和口腔系統(tǒng)Eye, ear, nose, throat and mouth system

              CAL-27細(xì)胞,人舌鱗癌細(xì)胞         5 × 105         1-2weeks

              Tca-8113細(xì)胞,     人舌鱗癌細(xì)胞         5 × 105         1-2weeks

              HDPC細(xì)胞,  人牙髓細(xì)胞     5 × 105         1-2weeks

                       人牙周膜細(xì)胞         5 × 105         1-2weeks

              PDLC細(xì)胞,   牙周膜成纖維細(xì)胞         5 × 105         1-2weeks

              CNE-2Z細(xì)胞,人鼻咽癌細(xì)胞        5 × 105         1-2weeks

                        人耳周膜細(xì)胞       5 × 105         1-2weeks

              SCC-9細(xì)胞,  人口腔癌細(xì)胞         5 × 105         1-2weeks

              KB細(xì)胞,人口腔表皮樣癌細(xì)胞   5 × 105         1-2weeks

              收到  處理方式:

              新鮮細(xì)胞到貨處理方式,客戶收到細(xì)胞后按以下方式操作:

              取出25cm2培養(yǎng)瓶,75%酒精消毒,拆下封口膜,放入37℃,5% CO2細(xì)胞培養(yǎng)箱中靜置2-3h,以穩(wěn)定細(xì)胞狀態(tài)。

              待細(xì)胞達(dá)到80%匯合時(shí)準(zhǔn)備進(jìn)行傳代培養(yǎng)。

              細(xì)胞傳代,吸出25cm2培養(yǎng)瓶中的培養(yǎng)基,用PBS清洗細(xì)胞一次;添加0.25%胰蛋白酶消化液約1ml至培養(yǎng)瓶中,37℃溫浴3min左右;倒置顯微鏡下觀察,待細(xì)胞回縮變圓后吸棄消化液,再加入*培養(yǎng)液終止消化;用吸管輕輕吹打混勻,按1:2適當(dāng)?shù)谋壤M(jìn)行接種傳代(原代細(xì)胞必須以1:2方式傳代),然后補(bǔ)充新鮮的*培養(yǎng)基至5ml,放入37℃,5% CO2細(xì)胞培養(yǎng)箱中培養(yǎng);待細(xì)胞*貼壁后,培養(yǎng)觀察。之后每隔2-3天更換新鮮的*培養(yǎng)基。

              凍存管到貨后處理方式,客戶收到細(xì)胞產(chǎn)品后,請(qǐng)按照下述方法操作:

              細(xì)胞凍存管公司多贈(zèng)送一管,建議客戶先復(fù)蘇1支,如果復(fù)蘇效果不理想,先與技術(shù)人員溝 通后再?gòu)?fù)蘇另1支。

              細(xì)胞收到后盡快復(fù)蘇,有效期為一周。

              收到凍存管后如不馬上復(fù)蘇,可以暫時(shí)放置于-80℃冰箱,不建議放入液氮罐。

              復(fù)蘇所用血清必須是胎牛血清。

              復(fù)蘇請(qǐng)按照細(xì)胞復(fù)蘇 SOP 方式操作(細(xì)胞復(fù)蘇 SOP 另行發(fā)送)。

               

              熱賣:DC2.4細(xì)胞,SW48細(xì)胞,RS4:11細(xì)胞,KGN細(xì)胞,A2780細(xì)胞,A2780/DDP細(xì)胞,HL-1細(xì)胞,COV434細(xì)胞等

              更多人卵巢癌細(xì)胞系、人肺癌細(xì)胞系、人結(jié)腸癌細(xì)胞系、人肝癌細(xì)胞系、人膀胱癌細(xì)胞系 耐藥株 盡在慧穎細(xì)胞庫(kù)

              訂購(gòu): 

              :  

                2452981082

              :王

              公司地址:上海市嘉定區(qū)眾仁路399號(hào)運(yùn)通星財(cái)富廣場(chǎng)A座202室

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