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中文名稱:人軟骨糖蛋白39(HCgp-39)Elisa試劑盒
英文名稱:Human GP39 (Glycoprotein 39, Cartilage/Chitinase 3-Like 1) ELISA Kit
規(guī)格:48人份/96人份,定量/定性
保存溫度:4°C/-20°C
有效期:6個月/12個月
適合樣本:血清、血漿等其它生物樣品
人軟骨糖蛋白39(HCgp-39)Elisa試劑盒 Human GP39 (Glycoprotein 39, Cartilage/Chitinase 3-Like 1) ELISA Kit 相關(guān)產(chǎn)品》》
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人軟骨糖蛋白39(HCgp-39)Elisa試劑盒檢測原理》》
本試劑盒采用雙抗體夾心ELISA法。用抗人GP39抗體包被于酶標板上,實驗時標本或標準品中的GP39會與包被抗體結(jié)合,游離的成分被洗去。依次加入生物素化的抗人GP39抗體和辣根過氧化物酶標記的親和素。抗人GP39抗體與結(jié)合在包被抗體上的人GP39結(jié)合、生物素與親和素特異性結(jié)合而形成免疫復(fù)合物,游離的成分被洗去。加入顯色底物,TMB在辣根過氧化物酶的催化下現(xiàn)藍色,加終止液后變黃。用酶標儀在450nm波長處測OD值,GP39濃度與OD450值之間呈正比,通過繪制標準曲線求出標本中GP39的濃度。
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