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潮霉素B(Hygromycin B)Roche老包裝,大量庫存,*,訂購: 56980388 !
產品名稱:潮霉素B
英文名稱:hygromycin B
品牌:Roche 老包裝
貨號:10843555001
規(guī)格:1G
市場價格:1933元
定義:潮霉素B由吸水鏈霉菌(Streptomyces hygroscopicus)產生的一種氨基糖苷類抗生素,通過抑制蛋白質合成能殺死細菌、真菌和高等真核細胞,用于篩選和保持轉化有潮霉素抗性基因的原核和真核細胞。
用途:
1)潮霉素B是氨基糖苷類抗生素,它可以通過抑制蛋白質合成殺死細菌、真菌和高級真核細胞。
2)潮霉素B通過干擾移位、促進80S核糖體的誤譯來抑制蛋白質合成。
3)哺乳動物細胞選擇的有效濃度通常是50 μg/ml--1 mg/ml之間;對于植物細胞,其有效濃度是20–200 μg/ml;對于細菌,有效濃度是20–200μg/ml;對于真菌,有效濃度是200μg–1 mg/ml。
4)轉染中用的特定抗生素。
5)潮霉素B磷酸轉移酶滅活潮霉素B。
6)攜帶hph基因轉染的細胞對潮霉素有抗性。
7)轉染細胞穩(wěn)定或暫時表達該基因。
產品描述:
Hygromycin B is an aminoglycosidic antibiotic that inhibits protein synthesis in prokaryotes and eukaryotes. It is used to select and maintain eukaryotic cells that are stably transfected with the E. coli hygromycin-resistance gene (hyg or hph).
For life science research only.
Product Description
Formula: C20H37N3O13
LD50: 6 mg/kg in mouse (i.v.);13 mg/kg in guinea pig (i.p.); 63 mg/kg in rat (i.p.)
Molecular weight: Mr = 527.5
Working concentration: 50 - 1,000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used.
Contents
Solution, 50 mg/ml, in PBS (phosphate-buffered saline), sterile-filtered
Quality
Purity: >80% (HPLC)
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